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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has long been observed in several tumors, which results in chromosome instability, and thus might contribute to tumorigenesis. This protein was discovered to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a important function in selling mobile-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have been described. Two additional splice variants have been reported but thorough nucleotide sequence info will not be yet accessible.

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Blocking peptides are peptides that bind specially to your focus on antibody and block antibody binding. These peptide commonly incorporates the epitope identified because of the antibody.

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This information outlines even more attainable optimization of cellular and immunoassay parameters to be sure the absolute best final results are...

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This guideline outlines even further achievable optimization of mobile and immunoassay parameters to be sure the very best final results are...

Antibodies bound to the blocking peptide not bind to the epitope about the concentrate on protein. By comparing the staining with the blocked antibody versus the antibody on your own, one can see which staining is precise.

Target Details Cyclin E belongs to your highly conserved cyclin loved ones, whose customers are characterised by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins operate as regulators of CDK kinases. Unique cyclins show unique expression and degradation designs which contribute into the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and functions to be a regulatory subunit of CDK2, whose activity is required for mobile cycle G1/S changeover. This protein accumulates within the G1-S stage boundary and it is degraded as cells progress by S period.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by each antibodies...

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